lab analysis

eDNA assays

Our metabarcoding panels have been developed to provide high taxonomic resolution and broad ecosystem scope, maximising quality and value. They can analyse samples collected with any of our eDNA sampling kits.

You should have your eDNA results within 3 weeks of Wilderlab receiving your samples

(Terms and conditions apply).

MARINE COMPREHENSIVE

FRESHWATER COMPREHENSIVE

SOIL/SCAT COMPREHENSIVE

We have two levels of eDNA metabarcoding analysis. For most requirements, a COMPREHENSIVE assay panel is most suitable. The BASIC option is also available for monitoring fish or for species identification.

eDNA analysis assays, by panel
Target Taxonomic Group Assay Code Gene Region Basic Comprehensive
Basic Panel Freshwater Freshwater Freshwater Marine Soil/scat
USA & elsewhere New Zealand* Australia
Vertebrates including fish, mammals, reptiles, amphibians, and birds WV mt16S Included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Vertebrates including fish, mammals, reptiles, amphibians, and birds RV mt12S Included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Vertebrates including fish, mammals, reptiles, amphibians, and birds LM mt12S Included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Fish (MiFish-U and MiFish-E combined assay) LX mt12S Included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Crustaceans including crayfish, crabs, prawns, and other arthropods HD mt16S Included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Freshwater insects and a range of other invertebrates CI COI Included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Microbes and microeukaryotes BU 18S-V9 Not included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Microbes UM 16S-V5 Not included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Venerid clams, including Corbicula WG mt16S Not included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Vascular plants TP trnL Not included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Vascular plants GV ITS1 Not included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Platypus XO D-loop Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Echyridella freshwater mussels EA COI Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Gobiomorphus bullies XG COI Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Oncorhynchus salmon XT COI Not included in Basic Panel Included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Corals, jellies, and sponges GD ITS2 Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Corals, jellies, and sponges GC ITS2 Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Corals, jellies, and sponges MA 28S Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Corals, jellies, and sponges MC 28S Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Included in Comprehensive Marine Not included in Comprehensive Soil, Specimen, Diet/Scat
Eukaryotes BX 18S Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Fungi GF ITS1 Not included in Basic Panel Not included in Comprehensive Freshwater — USA and elsewhere Not included in Comprehensive Freshwater — New Zealand Not included in Comprehensive Freshwater — Australia Not included in Comprehensive Marine Included in Comprehensive Soil, Specimen, Diet/Scat
Minimum number of assays on panel 6 12 13 12 13 12

*Includes ISO/IEC 17025 accredited TICI score.

Comprehensive Analysis

USD $182 ‍ ‍NZD $307 AUD $278 GBP £139‍

per sample (excluding tax and sampling kit).

Analysis service includes DNA extraction, sample QC, library preparation, Illumina single-end sequencing, and bioinformatic analysis including denoising, taxon assignment and data provision.

Our comprehensive metabarcoding panels provide a whole-ecosystem approach to biological monitoring, encompassing species across the tree of life. They offer all the coverage of the basic panel, with additional assays for detecting invertebrates, plants, algae, zooplankton and microorganisms. For New Zealand freshwater samples, this panel also includes the taxon-independent community index (TICI), a new and powerful way to track aquatic ecosystem health. 

A selection of comprehensive panels are available for different environment types:

  • Freshwater panels: suitable for rivers, streams, ponds, lakes, wetlands and estuaries. Choose the appropriate freshwater panel for your region.

  • Marine panel: suitable for coastal and offshore marine water and sediment samples

  • Soil, specimen, and scat panel: includes a specialised workflow and assay panel for inhibitor-rich samples such as soils, sediments, and animal diet analysis.

Basic Analysis

USD $114 ‍NZD $192 AUD $174 GBP £83‍

per sample (excluding tax and sampling kit).

Analysis service includes DNA extraction, sample QC, library preparation, Illumina single-end sequencing and bioinformatic analysis including denoising, taxonomic identification and data provision.

Our basic analysis panel includes six metabarcoding assays targeting fish, mammals, birds, reptiles, amphibians, and some inverts. It is tailored to provide a low-cost snapshot of aquatic animal diversity, and is suitable for specialised applications such as fish passage assessment, specimen identification, and lizard tracking tunnels.