Submitting Extracts to Wilderlab

Follow the guidelines below to ensure your extracts are compatible with our processing pipeline and meet the volume requirements for our assay panels options of Basic and Comprehensive

Plate format and sample preparation

• Sample requirement: Up to 94 extracts allowed on a plate (including controls) to allow for 2x Wilderlab internal controls. 

• Submission format: Submit extracts in barcoded ThermoFisher Matrix tubes. We will also accept plates with strips lids upon prior approval. Contact us.

  • Wilderlab will send empty racks of matrix tubes free of charge upon request. An 8-channel decapper can also be loaned out upon request. Please reach out for more information.

• Volume requirement: Each well must contain at least 80 µL of extract to run the full panels.

• Dilution: To reach volume requirement, extracts can be diluted using PCR-grade water or TE buffer (0.1 mM EDTA, e.g., the IDT formulation). Wilderlab will dilute

• Controls: Wilderlab adds a positive and negative control for PCR but we highly encourage including your own extraction controls on the same plate to provide context and quality assurance for your run.

Required documentation

To accompany your extracts, please ensure the following documentation is completed:

• Pre-extracted DNA template: Download the pre-extracted DNA template and fill in required metadata. Use our submission portal to submit the extracts metadata before sending the physical extracts to us. We will not be able to analyse the extracts until they have been submitted online.

• Buffer Safety Data Sheet: Include the current Safety Data Sheet (SDS) for the buffer used to store or dilute your extracts. This should match the buffer listed on the Manufacturer’s Declaration.

  • Place a clearly visible copy of the Manufacturer’s Declaration and SDS on the outside of the shipment and provide electronic copies to your courier where possible.

When extract preparation is complete view our information about Shipping Extracts to Wilderlab.